Theerlenmeyer weel ruxwaa weel dhaqameed gaar ah oo loogu talagalay dhaqanka unugyada laalista, waxaana sidoo kale loo isticmaali karaa diyaarinta, isku dhafka iyo kaydinta warbaahinta kala duwan.Dhaqankan la isticmaali karo waxaa loo adeegsadaa marka la dhaqan gelinayo unugyada Jurkat.

Khadka unugga ee Jurkat waxa uu ka yimi dhiigga wiil yar oo 14 jir ah waana unug cayrin ah.Xadhkaha unugyada ee ka soo jeeda Jurkat oo aan lahayn hiddo-sideyaal gaar ah ayaa durba laga helay bangiyada dhaqanka unugga.Khadadka T lymphocyte-ka ee bini'aadamka ee aan dhiman karin ayaa inta badan loo isticmaalaa in lagu barto leukemia-ga unugyada T-da ba'an, calaamadaha unugyada T-ga, iyo qaboojiyeyaasha kala duwan ee chemokine ee u nugul gelitaanka fayraska, gaar ahaan muujinta HIV.Waxay leedahay codsiyo kala duwan oo aad u ballaaran oo ku saabsan cilmi-baarista bayoolojiga, sida codsiga unugyada Jurkat si ay u bartaan M1-RNA ee ribonuclease P, iyo daraasadda M1-RNA ee anti-MHC fasalka II firfircoonida qoraalka (CIITA) si loo joojiyo muujinta molecules fasalka II ee MHC ee dusha unugyada.

Markaad ku dhaqanto unugyada Jurkat ee erlenmeyer shake flasks, RPMI1640 dhexdhexaad ah, 10% FBS ayaa loo baahan yahay;heerkulka waxaa lagu xakameeyaa at 37 ° C, 5% carbon dioxide, PH qiimaha 7.2-7.4, aseptic dhaqanka heerkulka joogto ah.Ku masax oo jeermi 75% aalkolo ka hor inta aanad u wareejin qolka fadhiga ee ultra-nadiif ah, ka soo saar cryovial unugga taangiga nitrogen dareeraha ah, isla markiiba ku rid biyaha qubeyska 37 ° C oo rux unugga cryotube si dhakhso ah si uu u dhalaaliyo.Ka dib, ka dib markii centrifugation, tuubooyinka iyo isku dhafka, iwm, waxaa la geliyey unug unug beerista.

Unugyadu waxay si gaar ah ugu nugul yihiin deegaanka.Marka lagu dhaqmo unugyada Jurkat ee weelasha ruxruxa unugga erlenmeyer, nadaafadda shakhsi ahaaneed waa in si wanaagsan loo sameeyaa, reagents nadiif ah waa in la isticmaalaa, iyo mabaadi'da hawlgalka aseptic waa in la raaco si looga fogaado soo bandhigida bakteeriyada iyo saameeya koritaanka unugyada.